Peak Extractor Instructions.
Multiple alignment file.
As described the 'Background' page of this web-site, the aligned
sequences must be preset in a single FASTA format file. All the
sequences must be of the same length. Gaps are represented by '-'.
The header, denaoted by '>' must be present on its own line
(no spaces), e.g.
Please note that the last nucleotides of sequences should be on the
last line of the file.
Choose the range of the global aligment you want to be extracted.
Specify the start and the end of the range of nucleotides you wish to
extract from the global alignment. For example, this information can be
obtained from a SynPlot plot. A web tool is available from this web site.
Select whether you want to see the extracted sequence in a plain text
format or formatted using PRETTYPLOT.
The extracted output can be retreived as a plain text file (in FASTA format).
This format can be used in other display utilities. However, the alignment can
also be formatted using the EMBOSS program PRETTYPLOT.
Select to view the consensus sequence for you alignment.
A consensus sequence may also be displayed with the extracted region.
Consensus nucleotides are selected if one nucleotide is present greater
than (number of sequences / 2) + 0.5 - rounded up to the next integer. These
nucletides corresspond to the blocks with a black background.
Specify whether the final PRETTYPLOT figure is in Postscript or PDF format.